In vitro antifungal activity of N-3-(1,2,4-dithiazole-5-thione)-resorcylcarbothioamide

Niewiadomy A, Krajewska-Kułak E, Łukaszuk C, Matysiak J, Kostecka M

The aim of the study was the determination of antifungal activity of N-3-(1,2,4-dithiazole-5-thione)-ß-resorcylcarbothioamide (DTRTA) against Candida albicans, non-Candida albicans, dermatophytes and molds and evaluation of the enzymatic activity C. albicans strains. We used reference strains C. albicans 10231 ATCC, 200 of C. albicans strains, 7 of non-C. albicans, 12 dermatophytes strains and 20 molds strains isolated from different ontocenoses from patients. DTRTA was synthesized at Department of Chemistry University of Agriculture in Lublin was used to tests. The mean MIC of DTRTA against C. albicans strains isolated from patients was 22.01 mg/L, for reference C. albicans 10 231 ATCC – 12.5 mg/L on Sabouraud’s medium (SB). The mean MIC of isolates from patients was 17.8 mg/L, and reference strains – 6.25 mg/L on YNB medium, respectively. The MICs of DTRTA against 7 non-C. albicans was 33 mg/L on SB and 18.2 mg/L on YNB. The MICs of DTRTA against dermatophytes ranged from 3 to 50 mg/L. The MICs of DTRTA against molds were 25 mg/L and 100 mg/L, respectively. C. albicans strains had the enzymatic activity of 16 among 19 hydrolases, after exposure to DTRTA, 15 among 19 enzymes, respectively. Non-C. albicans isolates had the enzymatic activity of 13 among 19 hydrolases, after exposure to DTRTA, 11 among 19 enzymes, respectively. This findings indicate hat DTRTA exerts a potent antifungal activity against the yeast-like fungi strains, dermatophytes and molds in vitro and.