Binding of 1-antichymotrypsin to the surface of lymphocytes – preliminary study

Sobieska M

Abstract

Purpose: The paper presents preliminary results of investigation of binding of 1-antichymotrypsin to the surface of peripheral blood lymphocytes.
Material and methods: Pooled serum samples from healthy individuals served as source of 1-antichymotrypsin for isolation using chromatography. Binding of 1-antichymotrypsin to the surface of peripheral blood lymphocytes was measured by flow cytometry.
Results: Even on native cells 1-antichymotrypsin may be detected. After incubation with isolated preparation the percentage of positive lymphocytes increased.
Conclusions: The presence of 1-antichymotrypsin on the surface of lymphocytes may imply its regulatory role during acute phase response and early immune response.

Key words: 	1-antichymotrypsin, lymphocytes, flow cyto­metry.