Serum isoprostanes levels in patients after abdominal hysterectomy

Szymczyk G, Bełtowski J, Marciniak A, Kotarski J

Abstract

Purpose: Reactive oxygen species (ROS), continuously generated in tissues, are involved in signal transduction under physiological conditions. The amount of ROS increases in response to surgical trauma. Isoprostanes are novel sensitive and specific markers of lipid peroxidation in vivo. Plasma concentration of isoprostanes increases in patients with various diseases associated with oxidative stress. In the present study we investigated the effect of abdominal hysterectomy on serum isoprostanes concentration.
Material and methods: The study was performed in 20 women (aged 45-63, average 50.3) who had undergone total abdominal hysterectomy with salpingooophorectomy, operated for benign diseases in the 1st Department of Gynaecology of Lublin Medical University. Isoprostanes were assayed by enzyme immunoassay (EIA) using 8-isoprostane EIA kit (Cayman Chemical, Ann Arbor, MI, USA).
Results: Serum concentration of isoprostanes before the surgery had value 38.9±10.7 pg/ml and it decreased at 8, 24 and 96 h after the operation.
Conclusions: Measurement of serum isoprostanes in small group of patients after hysterectomy did not brought the clear answer if the assessment of isoprostanes levels is a valuable method for evaluation of oxidative stress after a  surgery.

Key words: 	abdominal hysterectomy, isoprostanes, reactive oxygen species.