The choice of conditions for cathepsin D activity determination in human saliva

Roszkowska-Jakimiec W, D¹browska E, Gacko M, Karwowska A, Chlabicz M


The aim of the study was the demonstration and choice of conditions for the determination of cathepsin D activity in human mixed saliva. The 6% solution of hemoglobin, denatured with hydrochloric acid, was used as the substrate. The ratio of saliva volume to hemoglobin was 4:1 w/v. The reaction was interrupted by adding 10% trichloroacetic acid, after 6 hours of incubation at 37°C. The increase in degradation products was determined with the use of Folina and Ciocalteau method with copper modification.

Key words: human saliva, cathepsin D, method of determination, inhibitor from Vicia sativa L.